On 14th of December, at 14:00, there will be a seminar given by Pasi Tavi and William E. Louch. The talks are
Prof. Pasi Tavi: Cardiac effects of PGC-1alpha. Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland
Prof. William Edward Louch: Dyadic structure and function in cardiomyocytes: implications for heart failure.
Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, ...
Read More
Thesis defense: Martin Laasmaa
Martin Laasmaa is defending his thesis on Revealing Aspects of Cardiac Function from Fluorescence and Electrophysiological Recordings. Defense is on 14 December 2016 at 10:00 in the Institute of Cybernetics, room B101.
Read More
Revealing calcium fluxes by analyzing inhibition dynamics in action potential clamp
Laasmaa M, Birkedal R, Vendelin M
J. Mol. Cell. Cardiol. Volume 100, November 2016, Pages 93–108
PMID: 27702573
Full text: http://doi.org/10.1016/j.yjmcc.2016.08.015
Abstract
In cardiac excitation-contraction coupling (ECC), calcium enters the cytosol via L-type Ca2+ channels (LTCC) and reverse Na+/Ca2+-exchange (NCXrev), or is released from the sarcoplasmic reticulum (SR) by Ca2+-induced Ca2+-release (CICR). The magnitude of Ca2+ influx via the different pathways varies with the ...
Read More
Metabolic compartmentation in rainbow trout cardiomyocytes: coupling of hexokinase but not creatine kinase to mitochondrial respiration
Karro N, Sepp M, Jugai S, Laasmaa M, Vendelin M, Birkedal R
J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. 2016 Aug;
PMID: 27522222
Full text: http://doi.org/10.1007/s00360-016-1025-x
Abstract
Rainbow trout (Oncorhynchus mykiss) cardiomyocytes have a simple morphology with fewer membrane structures such as sarcoplasmic reticulum and t-tubules penetrating the cytosol. Despite this, intracellular ADP diffusion is restricted. Intriguingly, although diffusion is restricted, trout cardiom...
Read More
Restricted ADP movement in cardiomyocytes: Cytosolic diffusion obstacles are complemented with a small number of open mitochondrial voltage-dependent anion channels
Simson P, Jepihhina N, Laasmaa M, Peterson P, Birkedal R, Vendelin M
J. Mol. Cell. Cardiol. Volume 97, August 2016, Pages 197–203;
PMID: 27261153
Full text: http://doi.org/10.1016/j.yjmcc.2016.04.012
Abstract
Adequate intracellular energy transfer is crucial for proper cardiac function. In energy starved failing hearts, partial restoration of energy transfer can rescue mechanical performance. There are two types of diffusion obstacles that interfere with energy transfer from mit...
Read More
Thesis defense: Mari Kalda
Mari Kalda is defending her thesis on Mechanoenergetics of a Single Cardiomyocyte. Defense is on 10 December 2015 at 10:00 in the Institute of Cybernetics, room B101.
Read More
Cross-Bridge Group Ensembles Describing Cooperativity in Thermodynamically Consistent Way
Kalda M, Peterson P, Vendelin M
PLoS ONE 2015;10(9):e0137438
PMID: 26361396
Full text: http://doi.org/10.1371/journal.pone.0137438
Abstract
The aim of this work is to incorporate cooperativity into Huxley-type cross-bridge model in thermodynamically consistent way. While the Huxley-type models assume that cross-bridges act independently from each other, we take into account that each cross-bridge is influenced by its neighbors and cooperativity is induced by tropomyosin movement. For that...
Read More
Thesis defense: Niina Karro
Niina Karro is defending her thesis on Analysis of ADP Compartmentation in Cardiomyocytes and Its Role in Protection Against Mitochondrial Permeability Transition Pore Opening. Defense is on 5 October 2015 at 14:00 in the Institute of Cybernetics, room B101.
Read More
The location of energetic compartments affects energetic communication in cardiomyocytes
Birkedal R, Laasmaa M, Vendelin M
Front Physiol 2014;5:376
PMID: 25324784
Full text: http://dx.doi.org/10.3389/fphys.2014.00376
Abstract
The heart relies on accurate regulation of mitochondrial energy supply to match energy demand. The main regulators are Ca(2+) and feedback of ADP and Pi. Regulation via feedback has intrigued for decades. First, the heart exhibits a remarkable metabolic stability. Second, diffusion of ADP and other molecules is restricted specifically in heart an...
Read More
Tight coupling of Na+/K+-ATPase with glycolysis demonstrated in permeabilized rat cardiomyocytes
Sepp M, Sokolova N, Jugai S, Mandel M, Peterson P, Vendelin M
PLoS ONE 2014;9(6):e99413
PMID: 24932585
Abstract
The effective integrated organization of processes in cardiac cells is achieved, in part, by the functional compartmentation of energy transfer processes. Earlier, using permeabilized cardiomyocytes, we demonstrated the existence of tight coupling between some of cardiomyocyte ATPases and glycolysis in rat. In this work, we studied contribution of two membrane ATPases and w...
Read More