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Illaste A, Laasmaa M, Peterson P, Vendelin M Biophys. J. 2012 Feb;102(4):739-48 PMID: 22385844 Abstract Intracellular diffusion in muscle cells is known to be restricted. Although characteristics and localization of these restrictions is yet to be elucidated, it has been established that ischemia-reperfusion injury reduces the overall diffusion restriction. Here we apply an extended version…

Jepihhina N, Beraud N, Sepp M, Birkedal R, Vendelin M Biophys. J. 2011 Nov;101(9):2112-21 PMID: 22067148 Abstract Intracellular diffusion restrictions for ADP and other molecules have been predicted earlier based on experiments on permeabilized fibers or cardiomyocytes. However, it is possible that the effective diffusion distance is larger than the cell dimensions due to clumping…

Laasmaa M, Vendelin M, Peterson P J Microsc 2011 Aug;243(2):124-40 PMID: 21323670 Abstract Although confocal microscopes have considerably smaller contribution of out-of-focus light than widefield microscopes, the confocal images can still be enhanced mathematically if the optical and data acquisition effects are accounted for. For that, several deconvolution algorithms have been proposed. As a practical…

Birkedal R, Shiels HA, Vendelin M Am. J. Physiol., Cell Physiol. 2006 Dec;291(6):C1148-58 PMID: 16822946 Abstract We have developed a novel method to quantitatively analyze mitochondrial positioning in three dimensions. Using this method, we compared the relative positioning of mitochondria in adult rat and rainbow trout (Oncorhynchus mykiss) ventricular myocytes. Energetic data suggest that trout,…

Vendelin M, Béraud N, Guerrero K, Andrienko T, Kuznetsov AV, Olivares J, Kay L, Saks VA Am. J. Physiol., Cell Physiol. 2005 Mar;288(3):C757-67 PMID: 15496480 Abstract The aim of this work was to characterize quantitatively the arrangement of mitochondria in heart and skeletal muscles. We studied confocal images of mitochondria in nonfixed cardiomyocytes and fibers…