Measurement and interpretation of mitochondrial function and energy delivery in intact and permeabilized cells.
What we can do:
- High-resolution respirometry with controlled substrate and ADP titration
- Quantification of apparent ADP affinity and respiratory control
- NADH autofluorescence–based assessment of metabolic and redox state
- Analysis of intracellular diffusion barriers affecting ATP/ADP transfer
Typical problems this enables:
- Distinguishing mitochondrial capacity from energy delivery limitations
- Understanding energy starvation mechanisms in disease
- Evaluation of metabolic and mitochondrial drug effects in situ