General and specific keywords describing our research interests and methods.
GENERAL KEYWORDS
CORE RESEARCH AREAS
- Biophysics, Cardiac physiology, Heart disease, Cardiovascular medicine
- Bioenergetics, Cellular energetics, Metabolism
- Electrophysiology, Cell biology, Systems biology, Computational biology
- Skeletal muscle physiology, Muscle physiology
- Excitation-contraction coupling
- Calcium signaling, Intracellular signaling
- Mitochondrial biology, Oxidative phosphorylation
- Molecular transport, Cellular compartmentalization
- Comparative biology
DISEASE AREAS & THERAPEUTIC TARGETS
- Heart failure, Cardiac disease, Cardiovascular disorders
- Ischemic heart disease, Cardiac aging, Cardiomyopathy
- Metabolic diseases, Metabolic syndrome
- Muscle wasting disorders, Exercise physiology
- Cardioprotection, Therapeutic development
RARE DISEASES
- Creatine deficiency syndrome, Creatine metabolism disorders
- AGAT deficiency, Genetic metabolic disorders
- Rare genetic cardiomyopathies
MAJOR BIOLOGICAL PROCESSES
- Energy metabolism, Metabolic regulation, Metabolic coupling
- Cellular respiration, ATP synthesis, Energy transfer
- Energetic signaling
- Protein phosphorylation, Cell signaling pathways
- Calcium homeostasis, Cardiac contraction
- Molecular diffusion, Intracellular transport
- Gene expression regulation, Protein regulation
ADVANCED RESEARCH METHODS & TECHNOLOGIES
- Advanced microscopy, Custom microscope design, Live cell imaging, Super-resolution imaging
- Patch clamp, Respirometry, Single-cell analysis
- Fluorescence correlation spectroscopy (FCS), Raster image correlation spectroscopy (RICS)
- Computational modeling, Mathematical modeling
- Tissue engineering
STATISTICAL & COMPUTATIONAL APPROACHES
- Bayesian statistics, Statistical modeling
- Data integration
- Predictive modeling, Simulation methods
- Algorithm development, Software development
SEX & GENDER DIFFERENCES
- Sex differences in disease, Gender-specific medicine
- Precision medicine, Personalized medicine
- Hormonal influences, Sex-specific therapeutics
DEVELOPMENTAL & COMPARATIVE BIOLOGY
- Developmental biology, Cardiac development
- Comparative physiology, Evolutionary biology
- Model organisms, Animal models, Fish
TECHNOLOGY DEVELOPMENT
- Instrument development, Scientific software, Research tools development
- Database development, Data management
- Imaging technology, Analytical methods
- Open science, Reproducible research
- Laboratory automation
SPECIFIC KEYWORDS
CELLULAR BIOENERGETICS & METABOLISM
- ATP/ADP/AMP ratios, Phosphorylation potential, Energy debt
- Oxidative phosphorylation, Glycolytic capacity, Metabolic stability
- Creatine kinase system (MM-CK, MB-CK, BB-CK, Mis-CK)
- Adenylate kinase system (AK1, AK2, AK3, AK4)
- Phosphotransfer systems, PCr/Cr shuttle, Energy relay networks
- Metabolic coupling, Compartmentalized metabolism, Energy buffering
- Hexokinase activity and regulation (HK I, HK II)
- Pyruvate kinase coupling, Phosphoenolpyruvate (PEP) system
- Metabolic switching, Substrate channeling, Metabolic flexibility
- Fatty acid oxidation, Glucose metabolism, Citric acid cycle regulation
CARDIAC PHYSIOLOGY & EXCITATION-CONTRACTION COUPLING
- Cardiomyocyte isolation, Cardiac mechanics, Mechano-energetic coupling
- Calcium dynamics, Calcium sparks, Calcium transients, Calcium waves
- Sarcoplasmic reticulum (SR), Ryanodine receptors (RyR2)
- SERCA, Phospholamban (PLB), Calsequestrin
- L-type calcium channels (LTCC), Sodium-calcium exchanger (NCX)
- Action potential clamp, Electrophysiology, Patch clamp techniques
- β-adrenergic signaling, cAMP compartmentalization, PKA signaling
- Force-frequency relationships, Heart rate dependencies
- Excitation-contraction coupling maturation, Dyadic cleft function
CELLULAR STRUCTURE & COMPARTMENTALIZATION
- Sarcomeres, Myofibrils, Z-discs, M-bands, I-bands
- Transverse tubules (t-tubules), T-tubule orientation analysis
- Mitochondrial networks, Intermyofibrillar mitochondria
- Cellular architecture, Spatial organization, Diffusion barriers
- Macrocompartments, Microcompartments, Energetic compartmentalization
- Contact sites, Junctional membranes, Ca²⁺ release units (CRUs)
- Cytoskeletal framework, Microtubule networks
- VDAC (voltage-dependent anion channels), ANT (adenine nucleotide translocase)
ADVANCED MICROSCOPY & IMAGING TECHNIQUES
- Confocal microscopy, Super-resolution microscopy, dSTORM
- Raster image correlation spectroscopy (RICS)
- Fluorescence correlation spectroscopy (FCS)
- Single-molecule tracking, Live cell imaging
- Custom confocal microscope design, Automated image acquisition
- Point spread function characterization, Photon counting detectors
- 3D imaging, Calcium imaging, Fluorescence microscopy
- TIRF microscopy, Wide-field fluorescence imaging
MOLECULAR TRANSPORT & DIFFUSION
- Molecular diffusion, Anisotropic diffusion, Diffusion coefficients
- Diffusion restrictions, Lattice-like barriers, Size-dependent diffusion
- Molecular crowding effects, Bound vs free molecular fractions
- Intracellular transport, Motor proteins (kinesins, dyneins, myosins)
- Brownian motion, Directed transport, Protein trafficking
- Compartmentalized signaling, Local concentration gradients
COMPUTATIONAL BIOLOGY & MATHEMATICAL MODELING
- Reaction-diffusion equations, Michaelis-Menten kinetics modeling
- Multi-compartment metabolic models, Monte Carlo simulations
- 3D diffusion simulations, Finite element method (FEM)
- Stochastic modeling, Parameter optimization, Model validation
- Exchange coefficient determination, Nested model comparisons
- Bayesian analysis, Mathematical modeling of cellular processes
STATISTICAL ANALYSIS & DATA PROCESSING
- Bayesian statistics, Markov Chain Monte Carlo (MCMC)
- Statistical modeling, Posterior probability distributions
- Bayesian information criteria (BIC), Parameter estimation
- Linear mixed models, Cross-correlation analysis
- Signal processing, Time series analysis, Autocorrelation function
ANALYTICAL CHEMISTRY & BIOCHEMICAL METHODS
- Spectrophotometric enzyme assays, Coupled enzyme systems
- Western blotting, Gel electrophoresis, SDS-PAGE analysis
- Respirometry, Oxygen consumption measurements
- ADP titration protocols, Mitochondrial respiration kinetics
- Chemical inhibitor studies, Pharmacological analysis
- High-performance liquid chromatography (HPLC)
SOFTWARE DEVELOPMENT & DATA MANAGEMENT
- Scientific software development, Python programming
- Open-source software, Cross-platform development
- Database systems (SQLite, PostgreSQL), SQLAlchemy
- FAIR principles, Reproducible research, Metadata management
- IOCBIO software suite, Image analysis software
- Version control systems, Laboratory information management
DISEASE MODELS & PATHOPHYSIOLOGY
- Heart failure, Cardiac disease, Creatine deficiency syndrome
- AGAT knockout mice, GAMT knockout mice, Metabolic cardiomyopathy
- Ischemic heart disease, Cardiac aging, Muscle wasting disorders
- Transgenic mouse models
DEVELOPMENTAL BIOLOGY & COMPARATIVE PHYSIOLOGY
- Cardiac ontogeny, Neonatal vs adult cardiomyocytes
- Developmental transitions, Cardiomyocyte maturation
- Fish vs mammalian cardiac physiology, Species comparisons
- Temperature adaptation, Evolutionary adaptations
SEX DIFFERENCES & GENDER-SPECIFIC RESEARCH
- Sex differences in cardiac function, Male vs female cardiomyocytes
- Gender-specific cardiac disease, Sex hormones and cardiac function
- Oestrogen/testosterone effects on heart, Hormonal regulation
SYSTEMS BIOLOGY & INTERDISCIPLINARY APPROACHES
- Systems biology, Biophysics, Bioengineering
- Network organization, Structure-function relationships
- Hierarchical organization, Energetic signaling networks
- Applied mathematics, Computational methods