Positions
We are looking for postdoctoral fellows, PhD, MSc, and BSc students to join our team. For MSc and BSc students, an early start is recommended, as we work on exciting interdisciplinary subjects that may require learning different techniques and examining the physiology of the heart from several angles. See below for the list of proposed topics.
At present, the candidates for postdoctoral and other senior positions would have to apply for grants to finance their positions.
Please contact Marko Vendelin for further details.
Positions for PhD students
Get in touch if you are interested in joining. PhD positions are advertised centrally through the University. We usually also post them in the News section of our website. Please note that the website was recently redesigned, and older posts about previous PhD positions were not transferred to the new site.
Topics for BSc and MSc students
As the laboratory is interdisciplinary, we welcome students with different backgrounds, such as biology, chemistry, gene technology, physics, and IT.
The topics are given as a short outline and will be discussed and agreed upon with each candidate separately, depending on their interests and skills. The list will be updated over time, and feel free to express interest in any other topic within our expertise.
Unraveling the signaling pathways linking muscle energetics to phenotype.
Creatine kinase is important for energy transfer in muscle cells. Creatine-deficient mice exhibit changes in muscle metabolism and thus overall phenotype. This project aims at understanding the signaling pathways through which energetics affect the muscle phenotype.
How substrates affect mitochondrial function and regulation
The heart is considered a “metabolic omnivore”. This means that the heart in vivo uses a variety of substrates to fuel the production of ATP. This project addresses how different substrates affect mitochondrial function and regulation.
How mechanical contraction affects energetics in single cardiomyocytes
In the heart, electrical stimulation triggers contraction of cardiomyocytes. Using state-of-the-art techniques, we are able to measure the contractile force and determine how it varies under different conditions in single cardiomyocytes. This project addresses how changes in stimulation and mechanics affect the cell's energetics.
Calcium fluxes in cardiomyocytes
The contraction of the heart is regulated by multiple factors. Among other things, it is shaped by the calcium transient inside the cell. In this project, you will use a combination of electrophysiology and fluorescence microscopy to determine how the calcium fluxes are affected by different conditions.
Intracellular molecular movement
Within this topic, you will study how molecules move within the heart muscle cell. There are indications that the movement of the molecules is severely restricted in the heart muscle cell. The physical basis for that is not fully understood and is the aim of this research topic. Here, you will participate in the development of new methods based on fluorescence correlation spectroscopy. So far, we have built our own microscopes and designed new approaches to analyze the measured data. You will be able to work on the experimental parts of the study and data analysis.
Example studies using raster image correlation spectroscopy and mitochondrial response.
Processing of microscopy images
Optical microscopy is a tool frequently used to study live cells. Compared with other microscopy methods, it has the advantage of following a cell while it is alive, allowing us to study the cell's response to different stimuli. However, both diffraction-limited resolution and photon-detection noise affect the imaging. Within this topic, you will work on developing approaches to enhance the resolution and signal-to-noise ratio of recorded images.
Paper on deconvolution and corresponding software.
Mathematical modeling of the heart
Within this topic, you are expected to work on the state of the art mathematical models of cardiac function. Our earlier models include models of reaction-diffusion describing cardiac energetics, models of cardiac force generation, models of mitochondrial respiration, molecular dynamics model of proteins on the mitochondrial membrane, heart ventricle contraction, and energy consumption models.
Example models of cardiac contraction and molecular dynamics simulations.
Standards-based export of laboratory database data
We have developed a database-oriented approach in several software packages used to analyze experimental data. Within this topic, you would work on establishing or applying available software for exporting data from the laboratory database to external open databases. The export would need to describe the data and adapt it using standards-based approaches.