Cellular bioenergetics & intracellular energy transfer

Measurement and interpretation of mitochondrial function and energy delivery in intact and permeabilized cells. 

What we can do: 

• High-resolution respirometry with controlled substrate and ADP titration 

• Quantification of apparent ADP affinity and respiratory control 

• NADH autofluorescence–based assessment of metabolic and redox state 

• Analysis of intracellular diffusion barriers affecting ATP/ADP transfer 

Typical problems this enables: 

• Distinguishing mitochondrial capacity from energy delivery limitations 

• Understanding energy starvation mechanisms in disease 

• Evaluation of metabolic and mitochondrial drug effects in situ